Exploring protein oligomerization and stoichiometry with SEC-MALS: Case studies from phage-bacteria conflict
Presented by: Kevin Corbett, Ph.D., University of California, San Diego and Nayanthara Dharmaratne, Ph.D., Wyatt Technology
Presented Live: October 18, 2022
Newly available genomic data on diverse organisms, coupled with the development of new structural tools like cryo-electron microscopy and powerful structure prediction algorithms, has opened new frontiers in exploring novel biological pathways. A key step in understanding many new pathways is the identification and characterization of functional protein-protein complexes. This webinar summarizes the utility of SEC-MALS in characterizing oligomeric states of protein homo- and hetero-complexes, and show how these data complement different structural methods to drive fundamental discoveries. To illustrate each concept, the webinar will present case studies on bacteria-bacteriophage conflict.