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FFF 2.0: Obtaining high resolution distributions of nanoparticle size and zeta potential


Presented Live: July 24, 2018

Speakers: Christoph Johann, Ph.D.

This webinar will show how complex nanosuspensions, consisting of macromolecules and nanoparticles with sizes 1-1000 nm, can be characterized with high resolution using field-flow fractionation (FFF) coupled to a multi-angle light scattering (MALS) detector. In the life sciences and medicinal fields, FFF-MALS is beneficial for characterizing and isolating gene vectors, liposomal drug formulations, protein aggregates and exosomal biomarkers, but it is also useful in environmental and industrial applications such as lignocellulose, starch, latex paints and effluent or soil particulates.
The principle of separation by Flow-FFF and determination of size using MALS is presented. The FFF 2.0 workflow and novel software platform are demonstrated, from initial simulations and rationalized method refinement to the final, fully-optimized method with data analysis. In addition, the new method of determining electrophoretic mobility by Electrical/Asymmetric Flow-FFF (EAF4) is introduced, with examples to include particles and proteins.


  Webinar Q & A




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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.

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