Field-Flow Fractionation Combined with Multi-Angle Light Scattering

Presented by: Jeffrey A. Ahlgren, Ph.D., Senior Applications Scientist, Wyatt Technology
Presented Live: June 11, 2009

Field-Flow Fractionation (FFF) is a technique that fractionates macromolecular species using laminar flow without any stationary phase present. The advantage of this technique is that it can fractionate a broad range of sizes in a single run; by removing the stationary phase from the method, problems with sample absorption are frequently eliminated. When this technique is combined with downstream detection by multi-angle light scattering (MALS) and a concentration detector, the molar mass and size can be determined and true polydispersity of the sample can be evaluated. This webinar explains how the principle of FFF works and presents a wide variety of applications for FFF-MALS analysis.