Wyatt Technology

Measuring Size, Stability and Conformation of Biopolymers


Presented Live: March 23, 2016 and May 4, 2016

Speaker: John Champagne, Ph.D. Senior Applications Scientist and Northeast Regional Manager, Wyatt Technology

    Light scattering (LS), including classical and dynamic, has been widely employed to characterize protein solutions and other biomolecules. Classical or static light scattering, especially multi-angle light scattering (MALS), determines the absolute molecular weight of macromolecules in solution, facilitating the characterization of molecular heterogeneity and impurity profile. Dynamic light scattering (DLS), also known as quasi-elastic light scattering (QELS), directly measures the translational diffusion coefficient from which the hydrodynamic radius of a molecule is derived. Both static and dynamic light scattering can be used either in stand-alone (unfractionated, batch) mode or on-line coupled with a separation techniques, such as size exclusion chromatography (SEC) or field-flow fractionation (FFF).




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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.

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