Measuring Size, Stability and Conformation of Biopolymers with Dynamic Light Scattering

John Champagne

Presented by: John Champagne, Ph.D., Senior Applications Scientist and Northeast Regional Manager, Wyatt Technology
Presented Live: March 23, 2016 and May 4, 2016

Light scattering (LS), including classical and dynamic, has been widely employed to characterize protein solutions and other biomolecules. Classical or static light scattering, especially multi-angle light scattering (MALS), determines the absolute molecular weight of macromolecules in solution, facilitating the characterization of molecular heterogeneity and impurity profile. Dynamic light scattering (DLS), also known as quasi-elastic light scattering (QELS), directly measures the translational diffusion coefficient from which the hydrodynamic radius of a molecule is derived. Both static and dynamic light scattering can be used either in stand-alone (unfractionated, batch) mode or on-line coupled with a separation techniques, such as size exclusion chromatography (SEC) or field-flow fractionation (FFF).

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