Rational use of dynamic light scattering for the characterization of cytokines, bispecific antibody fusion proteins and binary antibody mixtures

Presented by: Hristo Svilenov, Ph.D., Technical University of Munich, and Sophia Kenrick, Ph.D., Wyatt Technology
Presented Live: November 22, 2020

The rational biophysical characterization of therapeutic protein candidates and their formulations can greatly improve the early-stage development process of new biologics. As a part of this characterization, dynamic light scattering (DLS) is a valuable technique that can be applied to understand the aggregation behavior and colloidal stability of proteins in solution.

This webinar will present three case studies in which DLS was crucial to understanding the stability of therapeutically relevant proteins. The first study shows how DLS in multi-well plates can quickly indicate which solution conditions are expected to suppress the aggregation of a cytokine during long-term storage. The second part of the presentation will focus on the characterization of a bispecific antibody fusion protein in different formulations, and how DLS-based formulation ranking correlates with the ranking based on aggregates formed after long-term storage. The third case study examines binary mixtures of monoclonal antibodies with different biophysical properties and how DLS helps investigate whether the antibodies mutually impair their physical stability in solution.

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