Robust and High-Resolution Characterization of Viral Vector Quality from Development to Release with Light Scattering Techniques
Presented by: Dr. Parker Lee, Waters | Wyatt Technology
Presented Live: December 3, 2024
Viral vectors continue to be critical in the development of new gene and cell therapies. However, in-depth characterization of these complex system remains a challenge throughout the development and manufacturing process. Developing robust analytical methodologies to characterize multiple critical quality attributes (CQAs) for large heterogeneous viral vector systems can be challenging.
In this webinar we will discuss how to implement high throughput dynamic light scattering (DLS) and electrophoretic lights scattering (ELS) workflows initial screening, along with and multi-angle light scattering combined with size exclusion chromatography (SEC-MALS) or field-flow fractionation (FFF-MALS) for in-depth simultaneous multiple CQA characterization of AAV, LV, and other gene delivery vectors. Additionally, we will explore how to implement light scattering into bioprocess unit operations for real-time CQA analysis. The CQAs for these techniques include size, size distribution, aggregation, concentration, stability, and capsid content.