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SEC-MALS for the Characterization of Complex Drugs and Sugar-Protein Complexes of Immunological Importance

 

Presented Live: November 4, 2015 at the 24th International Light Scattering Colloquium, Santa Barbara, CA

Speaker: Dr. Joseph Glajch, Director of Analytical Development at Momenta Pharmaceuticals

Abstract: Dr. Glajch’s plenary lecture details the use of multi-angle light scattering detection (MALS) in combination with an optimized SEC separation to more adequately characterize a complex polymeric, polypeptide mixture, Glatiramer Acetate. A comparison to more standard techniques is provided to demonstrate the advantage of SEC-MALS for these measurements. In addition, the use of SEC-MALS to monitor the reactions is illustrated. This presentation also reports on the use of SEC-MALS to study the size of complexes formed between heparin and PF4, which ranged from less than 100 kDa to over 50 MDa.

 

 

   

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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.



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