Using chemical denaturants and light scattering to determine aggregation propensity of biopharmaceuticals

Presented by: Luke Holloway, Ph.D., Wyatt Technology
Presented Live: October 27, 2021

Determining the developability of candidate molecules and optimizing formulations require rapid assessment of biophysical characteristics using accelerated stressors to represent normal storage conditions. Thermal stress, assuming the Arrhenius relationship between temperature and degradation rate (which has been successfully used for small molecule development), is the most common approach. Unfortunately, this relationship is not straightforward for biological molecules, so alternative approaches, such as using denaturants to accelerate non-native interactions, could prove beneficial.

Static and dynamic light scattering are invaluable tools for quantifying protein-protein interactions and aggregation phenomena. This webinar will review light scattering-based methods for protein characterization and explain their utility, when combined with partially denaturing buffers, in understanding aggregation propensity of monoclonal antibodies (mAbs).

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