Multi-Angle: August 2024
USP - Wyatt Technology Collaboration on SEC-MALS
USP’s characterization of commercial PLGA utilizing SEC-multi-angle light scattering and refractive index techniques via absolute method approach
Multi-angle light scattering coupled to size-exclusion chromatography (SEC-MALS) is commonly used to characterize the molecular weight of poly(lactic-co-glycolic acid) (PLGA). However, applying SEC-MALS as a validated method to analyze PLGA for drug delivery and biomedical device applications requires an additional level of methodological consistency. This study systematically explores the impact of SEC column selection, and of MALS analytical procedures such as baseline and peak range selection, on the precision and reliability of the resultant molar mass moments and polydispersity index.
Featured Customer
Connor A. Brandenburg, Takeda Pharmaceutical
Connor is a Sr. Research Associate in the Oligonucleotide Chemistry & Delivery group at Takeda Pharmaceutical Co. He has found LC-MALS to be a critical tool for characterizing antibody-oligonucleotides (AOC) and their stability in different formulation conditions.
Blog
Maximizing mAb product and process development with light scattering
Check out our blog on mAbs, highlighting their efficacy in treating diseases from cancer to rheumatoid arthritis. We share a brief overview of the intricate upstream and downstream processes of mAb production and the critical role of dynamic and multi-angle light scattering technologies in ensuring the quality and efficacy of these biopharmaceuticals.
Ask the Expert
How can I automate ZetaStar experiments?
Automating experiments increases productivity and enhances sample-to-sample consistency. Setting up a sample analysis sequence with your DynaPro ZetaStar instrument, an autosampler and HPLC CONNECT 4 software is simple and straightforward.
New Application Note
Streamline your LNP-mRNA quantification with FFF-MALS!
LNP-mRNA quantification can be a daunting task, requiring multiple tests, time, and specialized training, and causing a strain in development timelines. Field-flow fractionation coupled with multi-angle light scattering (FFF-MALS) is a multi-attribute technology that offers a more precise alternative to traditional dye-based assays, allowing for higher resolution without compromising integrity. Check out this application note to see how FFF-MALS can help simplify and expedite the formulation process through the quantification of two LNP-mRNA case studies.
Webinar: Live Webinar on Antibodies and XDCs
August 14th with Hongjia Liu, Ph.D. - Waters | Wyatt Technology
XDCs, such as antibody-drug conjugates (ADC), antibody oligo conjugates (AOC), ribonucleoproteins (RNP), and conjugate vaccines are growing rapidly due to advances in their component technology and their promise of higher efficacy compared to traditional therapies. In this online seminar, we will demonstrate the value of light scattering in XDC development and its potential application in quality control (QC) as part of a release assay.
Webinar: The Basics of DLS and MALS
August 28th with Udayabagya Halim, Ph.D. - Waters | Wyatt Technology
Biophysical techniques like multi-angle light scattering (MALS) and dynamic light scattering (DLS) are crucial in biotherapeutic formulation development for addressing aggregation, stability, viscosity, and chemical modifications. This webinar will cover the fundamentals and advantages of SEC-MALS and DLS for characterizing monoclonal antibodies (mAbs), conjugated proteins, and adeno-associated viruses (AAVs).
Webinar: Unveiling CQA Insights for mRNA, LNPs and Viral Vectors by SEC and MALS with Novel 1000 Å Ultrawide Pore Particle Columns
September 5th with Adam Crowe - Cytiva, Laveylay Kiezeki - Waters, and Mandana Fasth - Waters
Join our expert-led webinar on mastering light scattering techniques and applications. Dive into the principles of Wyatt light scattering technology. This session will cover mRNA applications and provide practical insights and real-world case studies. Don’t miss this opportunity to expand your knowledge.