AN1612: Protein PEGylation Processes Characterized by SEC-MALS

Since PEGylated proteins have a very different conformation, hydrodynamic size and molar mass relationship than globular proteins or linear polymers, they cannot be analyzed by standard analytical SEC using column calibration.

In this application note we demonstrate the information that can be obtained on the results of a PEGylation process by combining size-exclusion chromatography with multi-angle light scattering (SEC-MALS) to analyze conjugation.