Hemoglobin – Unusual SEC Elution Properties

Hemoglobin Molar Mass

When oxygen is released, carbon dioxide binds to hemoglobin to be transported out of the body. Human hemoglobin contains four polypeptides, in which the tetramer equilibrates with a dimer form, depending on the conditions. Using size-exclusion chromatography (SEC) in combination with multi-angle light scattering (MALS), a miniDAWN® detector and Optilab® DSP interferometric refractometer were used for the characterization.