AN2003: Quantifying AAV aggregation and quality attributes by FFF-MALS

Analysis of adeno-associated virus (AAV) with size exclusion chromatography and multi-angle light scattering (SEC-MALS) quantifies critical quality attributes (CQAs), such as particle concentration and capsid content. However, SEC-MALS is limited in analyzing large AAV aggregates. Field-flow fractionation combined with MALS (FFF-MALS) is an ideal technique for resolving and analyzing large AAV aggregates. Using Wyatt Technology’s Eclipse FFF-MALS system, both SEC-MALS and FFF-MALS may be performed with a single set of detectors, providing valuable orthogonal characterization of these gene therapy products. Learn more by reading our application note.