AN6601: Measuring size, concentration, and zeta potential of LNPs with the DynaPro ZetaStar

Microfluidic formulation of RNA-containing lipid nanoparticles (LNP-RNA) is a common means of developing and producing this important non-viral modality of gene delivery. A key challenge during formulation is the selection of the specific lipids and formulation conditions to achieve the desired particle sizes and surface potential. This application note demonstrates the use of the DynaPro™ ZetaStar™ instrument to rapidly characterize size, polydispersity and zeta potential of LNP-RNA suspensions, highlighting the outcomes of two different LNP formulation techniques.