AN8008: Real-time monitoring and control of AAV chromatographic enrichment with RT-MALS

Downstream purification and enrichment of full AAV capsids for gene therapy products is typically accomplished by ion-exchange chromatography (IEX). Real-time multi-angle light scattering (RT-MALS) quantifies biophysical attributes of AAVs including Vg/Cp, titer and aggregation. RT-MALS provides immediate results for critical quality attributes (CQAs) and enables optimized control over the collection of purified product.