Binding Stoichiometry of an Antibody Fragment

To determine the binding stoichiometry and the oligomeric state of the selected Fab fragment in complex with its target, we have used the powerful method of multi-angle light scattering in combination with size exclusion chromatography (SEC-MALS). This information is instrumental in further biophysical studies and co-crystallization experiments. Additional information such as weight fraction of protein and detergent in the protein-detergent micelle are obtained in the same experiment when using both UV and RI-detectors.