Wyatt Technology
  • Home
  • Understanding Monoclonal Antibody Unfolding and Aggrega ...

Understanding Monoclonal Antibody Unfolding and Aggregation


Presented Live: March 14, 2018

Speakers: Wolfgang Friess, Ph.D.

Investigations on thermal behavior are essential during the development of therapeutic proteins. Understanding the link between thermal unfolding and aggregation might help to minimize conformational and colloidal instabilities. In this study, a therapeutic monoclonal antibody (MAb) and its Fab and Fc fragments were investigated by differential scanning fluorimetry, temperature-ramped dynamic light scattering and turbidity measurement. Repulsive net charges at the low pH increased the colloidal stability, although a reduction of the conformational stability was observed. Aggregation was driven by hydrophobic interactions. Thus, the presented methods described and explained the thermal behavior of the protein and demonstrated their value for the development of pharmaceutical protein products. Coupling of differently charged hydrophobic payloads affected colloidal stability mostly based on the charge effects. The combination of orthogonal methods allows a better understanding of the conformational and colloidal stability of mAbs and mAb-conjugates.


  Webinar Q & A




Ultimate Guide

Thirteen key questions to ask when buying a Multi-Angle Light Scattering (MALS) or Dynamic Light Scattering (DLS) instrument.

Get Ultimate Guide


Solutions Catalog

Our Solutions Catalog combines Wyatt's product lines, applications and services into a single comprehensive booklet.

Request a Catalog




Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.

© Wyatt Technology Corporation. All rights reserved.