Protein-Protein Interactions: Scaling-up a Purification Protocol

Cellular proteins that are part of the protein synthesis machinery are often up-regulated under such conditions. Large quantities of the recombinant protein can be bound to these cellular proteins, making purification difficult. Analytical size exclusion chromatography, followed by on-line direct mass detection by multi-angle light scattering (MALS), showed that a significant amount of the recombinant protein was not bound to the rotamase.