Analysis of viral proteins, monoclonal antibodies and their interactions with light scattering

Felix Gloge

Presented by: Felix Gloge, Ph.D., Wyatt Technology
Presented Live: June 23, 2020

Characterizing viral proteins and their interactions with receptors and engineered monoclonal antibodies is key to fundamental understanding of how a virus infects cells and ultimately to the successful development of protein-based drugs. Light-scattering based methods provide essential solutions to addressing these questions.

Dynamic light scattering (DLS) offers rapid, low-volume assessment of protein quality and binding. Size-exclusion chromatography coupled to multi-angle light-scattering (SEC-MALS) delivers crucial information regarding protein quality and homogeneity, degree of glycosylation and complex formation. Composition-gradient light scattering (CG-MALS), which does not involve separation, is a label-free method for analyzing binding affinities between molecules in solution, and provides detailed information about the nature of homo- and hetero-oligomers. This webinar will shed light on the theory and technology of light scattering, and how it is used for the biophysical characterization of viral proteins, mAbs and their interactions.

Q&A Webinar Q & A