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Crystallized Biotherapeutics: Optimizing mAb Crystallization using Light Scattering

 

Presented Live: May 15, 2018

Speakers: Lynette Schroeter-Tedesco and Jonathan Alvarez

The Crystalomics® Department at Ajinomoto Althea CMO uses crystallized biotherapeutics to develop high concentration, low viscosity injectable drug formulations exceeding 200 mg/mL. The first step in our technology is to discover and develop crystallization conditions as rapidly as possible in order to meet short development timelines.

Crystallization of proteins, especially monoclonal antibodies (mAbs), is very challenging and typically employs extensive trial-and-error. Incorporating high-throughput dynamic light scattering (HT-DLS) into our pre-crystallization screening to characterize the protein in multiple buffers, we are able to significantly reduce the extent of crystallization trials. Analysis of the mAbs by multi-angle light scattering coupled to size-exclusion chromatography (SEC-MALS) as well as HT-DLS is critical to determining optimal crystallization conditions with minimal time and effort.

 

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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.



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