Wyatt Technology

Multi-Angle and Dynamic Light Scattering:
Essential tools for characterizing proteins, polymers, nanoparticles and more

What Can I Measure?

molar mass molecular weight
molecular nanoparticle size
zeta potential molecular charge
biomolecular interactions
polymer conformation nanoparticle shape
protein conjugation copolymer ratio

What Can I Analyze?

  • Biotherapeutics
  • Proteins
  • Biopolymers
  • Synthetic Polymers
  • Nanoparticles
  • Aggregation, sec-mals, fff-mals, cg-mals, dls

     

    Aggregation

    While SEC-MALS with a DAWN® or miniDAWN® is the primary means of characterizing soluble protein aggregates, FFF-MALS, CG-MALS and DLS are valuable orthogonal techniques to meet FDA requirements.

    Conjugation

     

    Conjugation

    SEC or FFF plus triple detection UV-MALS-RI and ASTRA® software determine the molar mass of protein and modifier in conjugated biotherapeutics such as antibody-drug conjugates or PEGylated proteins.

    Formulation

     

    Formulation

    The DynaPro® Plate Reader performs high-throughput screening of excipients and buffers for optimal formulation in situ in standard microwell plates, detecting aggregation as well as conformational and colloidal stability indicators.

    Interaction

     

    Interaction

    CG-MALS combines a Calypso® and DAWN or miniDAWN to characterize the affinity and absolute molecular stoichiometry of drug-target binding, without labeling or immobilization. It also analyzes self-association at low, moderate and high concentrations.

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  • Identification

     

    Identification

    A DAWN® or miniDAWN® MALS detector, combined with a Wyatt SEC column and the Optilab® dRI detector, determine absolute molar masses and sizes of peptides, proteins and other biomacromolecules independently of column calibration.

    Purification-well-plate

     

    Purification

    Validate protein purification and stability quickly and easily with a DynaPro® NanoStar® cuvette-based, combined dynamic light scattering & static light scattering detector, or a high-throughput DynaPro® Plate Reader.

    protein-charge-mobius-dip-cell

     

    Charge

    The Mobius® is the only electrophoretic light scattering detector capable of providing reliable and repeatable measurements of a protein's net charge in high-salt buffers.

    Binding

     

    Binding

    CG-MALS combines a Calypso® and DAWN or miniDAWN to characterize the affinity and absolute molecular stoichiometry of simple or complex biomolecular interactions. CG-MALS requires no labeling or immobilization, addressing self-association as well as hetero-association.

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  • Absolute Molar Mass & Size DAWN HELEOS II Optilab T-rEX

     

    Absolute Molar Mass and Size

    A DAWN® MALS detector and Optilab® dRI detector work together to determine distributions of absolute molar mass and size of natural or synthetic polymers independently of column calibration.

    Biodegradation

     

    Biodegradation

    Light scattering is an excellent means for assessing the degradation of biopolymers as a result of exposure to heat, light, high or low pH, and other stimuli.

    Polysaccharides

     

    Polysaccharides

    Key ingredients in cosmetics, the paper industry and food products, polysaccharides are also critical materials in biomedical applications such as vaccines and blood coagulants. Characterize all types of polysaccharides by SEC combined with Wyatt's on-line light scattering, dRI and viscosity detectors.

    Paper & Pulp

     

    Paper and Pulp

    Lignin and cellulose present some of the most challenging biopolymeric analytes due to their unique optical and morphological properties. Wyatt offers multiple solutions for meeting these challenges.

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  • Absolute Molar Mass & Size DAWN HELEOS II Optilab T-rEX

     

    Absolute Molar Mass and Size

    A DAWN® MALS detector and Optilab® dRI detector work together to determine distributions of absolute molar mass and size of natural or synthetic polymers independently of column calibration.

    Branching and Conformation

     

    Branching and Conformation

    Incorporate a WyattQELS™ DLS module and/or a ViscoStar® differential viscometer to characterize the branching ratio and conformation of complex polymers.

    FFF, Field-Flow Fractionation

     

    Field-Flow Fractionation

    The Eclipse® DualTec® provides true hydrodynamic fractionation without the stress, surface interactions and non-ideal separation present in SEC. Integrates perfectly with Wyatt MALS, dRI, DLS and viscometry detectors.

    dn/dc and zimm plot

     

    dn/dc and
    Zimm plots

    Wyatt offers several options to simplify laborious, error-prone dn/dc and Zimm analyses for natural and synthetic polymers.

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  • FFF, field flow fractionation

     

    Accurate Size Distributions

    Accurate size distributions with true ensemble representation require fractionation + independent, on-line determination of size. A DAWN® / Eclipse® FFF-MALS system can characterize simple or complex fluids incorporating macromolecules with large and small nanoparticles.

    Conformation & Composition

     

    Conformation and Composition

    Information about nanoparticle shape and structure can be obtained by combining MALS analysis with dynamic light scattering. The WyattQELS™ DLS module integrates into the DAWN® or miniDAWN® for simultaneous MALS and DLS analysis. Add a UV, fluorescence and/or Optilab® dRI detector for compositional studies.

    Formulation

     

    High-Throughput Sizing

    Need to characterize your nanoparticles under hundreds of solvent conditions in the space of a few hours? The DynaPro® Plate Reader measures size distributions by DLS, in situ in standard 96-, 384- or 1536-well microtiter plates. A camera conveniently photographs each well to identify precipitates.

    interaction zeta potential

     

    Zeta Potential / Charge

    The Mobius® is the only zeta potential detector capable of hands-free, high-throughput measurements by coupling to an autosampler. It measures charge and size simultaneously to monitor aggregation or degradation caused by the applied electric field.

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Ultimate Guide

Thirteen key questions to ask when buying a Multi-Angle Light Scattering (MALS) or Dynamic Light Scattering (DLS) instrument.

Get Ultimate Guide

   

Solutions Catalog

Our Solutions Catalog combines Wyatt's product lines, applications and services into a single comprehensive booklet.

Request a Catalog

 

 

   

Resources


Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.



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