Wyatt Technology

DC Regional Light Scattering User Meeting & Workshop

DC Region Protein & Biotech User Meeting

Join us in DC!
September 11th User Meeting: Advanced Topics in Light Scattering
September 12th Workshop: Light Scattering Characterization

User Meeting Registration

Light Scattering User Meetings

Wyatt's regional user meetings are a great opportunity to meet and interact with other scientists who utilize Wyatt light scattering instruments. Learn how your colleagues approach biophysical characterization, pick up some tips and tricks on method development, and spend some quality time with Wyatt staff. Registration is free for this event.

Light Scattering Workshop

New to light scattering? Wondering how light scattering works and how it’s applied? Wyatt's regional light scattering workshops are a wonderful opportunity to learn the “basics” of light scattering and see live demonstrations of Wyatt instruments. Learn the limits and capabilities of light scattering and how these methods can complement your existing suite of tools. Registration is free for this event.

Check back soon for Speakers and Agenda!
Details:
Date: September 11, 2019: User Meeting: Advanced Topics in Light Scattering
Date: September 12, 2019: Workshop: Light Scattering Characterization
Time: 8:30 AM
Location: Bethesda Marriott
5151 Pooks Hill Road
Bethesda Maryland 20814

Who should attend:
  • User Meeting: Active users of Wyatt light scattering instruments seeking to engage with regional colleagues around similar analytical challenges, and to learn more about advanced topics related to Wyatt instruments and software.

  • Workshop: Anyone interested in learning more about light scattering and its manifold applications to biomolecular, polymer and nanoparticle characterization. Note that space is limited for these events, please register early.
User Meetings: What’s covered:

In addition to guest speakers, this meeting will address several advanced topics presented by Wyatt scientists. Topics change from meeting to meeting, and may include:

  • Protein Conjugate Analysis
  • Co-polymer Analysis
  • ASTRA 7 Models: Zimm, Berry, or Debye
  • The Shape Factor applied to biomolecular and NP characterization
  • How to measure molar mass with the DynaPro Plate Reader
  • Analysis of Polymer branching
  • ASTRA and DYNAMICS SQL Database: Not just for 21CFR11
  • Determination of size, size distributions of nanoparticles
  • Formulation and stability of biotherapeutics
  • Characterizing vaccines, viruses and VLPs
  • Detection and quantification of aggregates, large and small
  • Analyzing biomolecular interactions

What’s not covered: Theoretical concepts underpinning the light scattering toolkit, including: SEC-MALS, FFF-MALS, CG-MALS, Dynamic Light Scattering, Electrophoretic Light Scattering are covered in the Wyatt Light Scattering Workshop that accompanies the user meeting.

Workshops: What’s covered:

The workshop will cover the basic theory of light scattering and related methods, followed by a series of workshops incorporating Wyatt instruments and software. Topics include:

  • The Light Scattering Toolbox Theory:
    • Dynamic Light Scattering (DLS)
    • Phase Analysis Light Scattering (PALS)
    • Multi-angle Light Scattering (MALS)
    • Asymmetric Flow Field Flow Fractionation (AF4)
    • Intrinsic Viscometry; and others
  • Work Stations may include:
    • High Throughput Automated DLS/SLS (DynaPro Plate Reader)
    • Batch DLS, SLS, and Zeta Potential (NanoStar, Mobius)
    • SEC-UV-MALS-RI (DAWN, Optilab)
    • SEC-MALS-RI-IV (DAWN, ViscoStar, Optilab)
    • AF4 (Eclipse)
    • CG-MALS (Calypso)
    Pricing:

    Registration is free. Continental breakfast and lunch are provided.

    Check back soon for Speakers and Agenda!

    User Meeting Registration


    September 2018 User Meeting was held in Bethesda, MD

    DC Region User Meeting Agenda

    Speakers
    • Ashwinkumar Bhirde, FDA\CDER\OPQ\OBP\DBRR2
      "Innovative Way of Screening Therapeutic Proteins for In-use Stability"

    • Allen Minton, NIDDK/NIH
      "High Throughput Quantitative Characterization of Thermodynamic & Colloidal Stability of Proteins with the DynaPro Plate Reader"

    • Andre Striegel, National Institute of Standards and Technology
      "Multi-Detector Hollow-Fiber Flow Field-Flow Fractionation (HF5) of Polysaccharides"

     


    September 2017 User Meeting was held in Gaithersburg, MD

    SF Bay User Meeting Agenda

    Speakers
    • Ashwinkumar Bhirde, FDA/CDER
      “High-throughput Screening of Colloidal Nanoparticles Under Simulated Physiological and Therapeutic Conditions”

    • Arun Parupudi, MedImmune Inc.
      “Light Scattering an Indispensable Technique for the Biopharmaceutical Industry”

     


    April 2014 Meeting was held in Gaithersburg, MD

    DC User Meeting Agenda

    Speakers
    • Lisa Kueltzo, NIH/NIAID Vaccine Research Center
      "Dynamic Light Scattering in Formulation Screening: Applications and Challenges"

    • Chris Rowe, NIH/NIAID Div. of Intramural Research
      "SEC-MALS-DLS for Malaria Conjugate Vaccines: From Bench to Clinic"

    • Allen Minton, NIH/NIDDK Lab. of Biochemistry and Genetics
      "Using DLS to Detect and Quantify the Effect of Osmolytes on Proteins Associations"

    • Di Wu, NIH/NIDDK Lab. of Biochemistry and Genetics
      "Using SLS to Detect and Quantify Nonspecific Protein-Protein Interactions"

    • Reza Esfandiary, MedImmune/Dept. of Formulation Sciences
      "Application of Light Scattering Techniques in Studying Protein-Protein Interactions: A Monoclonal Antibody Case Study"

    • Dan Some, Wyatt Technology
      "ADC Characterization: Molar Mass, Size, Conjugation and Aggregation" and
      "CG-MALS Case Study and How-To: Multi-Valent Protein Ligand Binding"

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    Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

    Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.



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