Crystalline Eye Lens Proteins: Conformational Changes

Protein biochemists often need to know the molar mass distributions and conformation of their samples. Using conventional GPC/SEC techniques, it is impossible to determine conformation because molar mass is assumed to decrease monotonically with elution volume. This application note reveals how you can determine conformation, and conformational changes by coupling a MALS detector (either a DAWN^® or miniDAWN^®) to the liquid chromatograph.