WP8003: IEX-MALS for protein separation and characterization

Ion-exchange chromatography coupled to multi-angle light scattering (IEX-MALS) allows absolute molar mass determination of proteins, protein complexes and peptides in a heterogenous sample. IEX-MALS can overcome some of the restrictions of SEC-MALS that arise from the limited separation ability of analytical SEC column.

The IEX-MALS method is valuable for quality assessment as well as characterization of native oligomers, charge variants, modified proteins and mixed-protein samples.