Request: Exploring protein oligomerization and stoichiometry with SEC-MALS: Case studies from phage-bacteria conflict

Key Learning Objectives:

• Understand the basic principles of SEC-MALS and its application to purified proteins in aqueous solution
• Explore the use of fusion tags and protein engineering to understand protein oligomerization
• Learn how SEC-MALS can complement structure determination tools like X-ray crystallography and cryo-electron microscopy
• Learn how SEC-MALS can complement structure prediction tools like AlphaFold and RosettaFold