Exploring protein oligomerization and stoichiometry with SEC-MALS: Case studies from phage-bacteria conflict request

Key Learning Objectives:

  • Understand the basic principles of SEC-MALS and its application to purified proteins in aqueous solution
  • Explore the use of fusion tags and protein engineering to understand protein oligomerization
  • Learn how SEC-MALS can complement structure determination tools like X-ray crystallography and cryo-electron microscopy
  • Learn how SEC-MALS can complement structure prediction tools like AlphaFold and RosettaFold