Wyatt Technology

Field-Flow Fractionation

  • Eclipse DualTec

    Eclipse DualTec

    Unprecedented productivity, and flexibility for separating proteins, polymers, colloids and nanoparticles.

    The Eclipse™ DualTec supports both Hollow-Fiber Flow-FFF (HF5) and Asymmetric Flow FFF (AF4) in one instrument. Utilizing one HPLC stack and one set of Wyatt detectors, the DualTec permits automated switching between any two of SEC, AF4 and HF5 separation followed by MALS and/or DLS characterization.

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  • Eclipse AF4

    Eclipse AF4

    A versatile system for separating macromolecules, proteins, colloids, and nanoparticles.

    The Eclipse™ AF4 is optimal for all standard asymmetric-flow field-flow fractionation (AF4) separations as well as fritless channels and semi-preparative AF4. Combined with industry-leading HPLC equipment and Wyatt's unique multi-angle light scattering detectors, the Eclipse AF4 excels at characterizing difficult samples.

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FFF System Comparison

Capabilities Eclipse™ DualTec™ Eclipse™ AF4
Channel Options    
Flat Channels (AF4)
  • Short SC: 153 mm e.l.
  • Long LC: 246 mm e.l
  • Short SC: 153 mm e.l.
  • Long LC: 246 mm e.l
  • Semi-Prep SP3: 246 mm e.l.
  • Frit-inlet Long Channel (Fl-AF4)
Hollow-Fiber Channels (HF5) Disposable, ready-to-use hollow-fiber cartridges: 400 µm radius, PES membrane 10 kDa cutoff, channel volume 90 µL No
Switching Automatic switching between two separation modes Optional module for switching between two separation modes
Metal-free version Yes No
Organic version Yes Yes


See Accessories for FFF



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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.

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