Unprecedented productivity, and flexibility for separating proteins, polymers, colloids and nanoparticles.
The Eclipse® DualTec supports both Hollow-Fiber Flow-FFF (HF5) and Asymmetric Flow FFF (AF4) in one instrument. Utilizing one HPLC stack and one set of Wyatt detectors, the DualTec permits automated switching between any two of SEC, AF4 and HF5 separation followed by MALS and/or DLS characterization.
A versatile system for separating macromolecules, proteins, colloids, and nanoparticles.
The Eclipse® AF4 is optimal for all standard asymmetric-flow field-flow fractionation (AF4) separations as well as fritless channels and semi-preparative AF4. Combined with industry-leading HPLC equipment and Wyatt's unique multi-angle light scattering detectors, the Eclipse AF4 excels at characterizing difficult samples.
FFF System Comparison
|Capabilities||Eclipse® DualTec™||Eclipse® AF4|
|Flat Channels (AF4)||
|Hollow-Fiber Channels (HF5)||Disposable, ready-to-use hollow-fiber cartridges: 400 µm radius, PES membrane 10 kDa cutoff, channel volume 90 µL||No|
|Mobility Electrical AF4 Channel (EAF4)||Yes||Yes|
|Switching||Automatic switching between two separation modes||Optional module for switching between two separation modes|