Fluorescent macromolecules pose an interesting challenge for analytical methods based on light scattering as the light emitted by the samples themselves can be detected as if it were scattered light. Learn what you may need to consider before working with fluorescing samples.
To accurately calculate the molar mass of a sample, we need to account for any excess light (fluorescence) or a decrease in the intensity of the laser source (absorbance) so these changes aren’t incorrectly attributed to the sample being analyzed. Learn more here.
Did you know that ASTRA® can be used to assess the quality of chromatography with statistics such as peak height, column plate count, peak width, asymmetry, and resolution? In fact, this analysis is built into every online method in the software so there’s no need to use your chromatography software to perform these calculations. This is particularly advantageous when using Wyatt’s HPLC Service to control your HPLC hardware or performing FFF-MALS experiments using VISION.
Unsure if your Wyatt product can be run right now? Check this support article for more details.
Collecting multiple UV wavelengths in ASTRA is useful for processing experiments where the sample contains two separate components that absorb at different wavelengths. Learn how here.
To accurately determine your sample’s molar mass, ASTRA needs information about the solvent. Learn how to add solvent information here.
Wyatt Technology’s HPLC Service allows ASTRA to control your HPLC or UHPLC, eliminating the need to enter sample information in a third-party application. Learn how to convert your existing methods to HPLC Control.
Running samples overnight or during the weekend can help to improve the throughput of your experiments, but there are some considerations to make when leaving a system unattended. Learn how to make sure your instruments shut down properly here.
ASTRA has a convenient One-to-Many feature that allows you to apply a method or some aspect of a method to multiple experiments. Learn more here.
Learn how to determine molar mass, size, and relative fractions of a copolymer or protein conjugate with light scattering.
Wyatt detectors enable experiments using a broad range of solvents and mobile phases. However, when you switch mobile phases you’ll need to revalidate your system with an appropriate molecular standard. Learn more here.