November 4, 2019

What causes a “ghost” peak and what can I do to prevent this?

September 19, 2019

What are some considerations and strategies when working with fluorescing samples?

Fluorescent macromolecules pose an interesting challenge for analytical methods based on light scattering as the light emitted by the samples themselves can be detected as if it were scattered light. Learn what you may need to consider before working with fluorescing samples.

August 20, 2019

How can I tell if I have fluorescing or absorbing samples?

To accurately calculate the molar mass of a sample, we need to account for any excess light (fluorescence) or a decrease in the intensity of the laser source (absorbance) so these changes aren’t incorrectly attributed to the sample being analyzed. Learn more here.

July 31, 2019

How can I assess the quality of my chromatography in ASTRA?

Did you know that ASTRA® can be used to assess the quality of chromatography with statistics such as peak height, column plate count, peak width, asymmetry, and resolution? In fact, this analysis is built into every online method in the software so there’s no need to use your chromatography software to perform these calculations. This is particularly advantageous when using Wyatt’s HPLC Service to control your HPLC hardware or performing FFF-MALS experiments using VISION.

June 10, 2019

How do the next generation of Wyatt instruments assess whether my system is ready to run?

Unsure if your Wyatt product can be run right now? Check this support article for more details.

June 8, 2019

I need to monitor multiple UV wavelengths for my sample. How can I do this with ASTRA?

Collecting multiple UV wavelengths in ASTRA is useful for processing experiments where the sample contains two separate components that absorb at different wavelengths. Learn how here.

June 7, 2019

I’m using a new solvent for my SEC-MALS runs. How do I add this solvent information to ASTRA?

To accurately determine your sample’s molar mass, ASTRA needs information about the solvent. Learn how to add solvent information here.

June 6, 2019

I recently added Wyatt’s HPLC Service software. How do I convert my existing methods to HPLC Control in ASTRA?

Wyatt Technology’s HPLC Service allows ASTRA to control your HPLC or UHPLC, eliminating the need to enter sample information in a third-party application. Learn how to convert your existing methods to HPLC Control.

June 5, 2019

I plan to run my samples over the weekend. How can I have the instruments shutdown after the run completes if I’m not there?

Running samples overnight or during the weekend can help to improve the throughput of your experiments, but there are some considerations to make when leaving a system unattended. Learn how to make sure your instruments shut down properly here.

June 3, 2019

I made a change to how I process my experiments. Is there a way to easily apply this change to my existing experiments?

ASTRA has a convenient One-to-Many feature that allows you to apply a method or some aspect of a method to multiple experiments. Learn more here.

June 2, 2019

Is it possible to determine molar mass, size, and relative fractions of a copolymer or protein conjugate with light scattering?

Learn how to determine molar mass, size, and relative fractions of a copolymer or protein conjugate with light scattering.

June 1, 2019

I found the perfect mobile phase for my experiments. Now how do I find the right validation standard?

Wyatt detectors enable experiments using a broad range of solvents and mobile phases. However, when you switch mobile phases you’ll need to revalidate your system with an appropriate molecular standard. Learn more here.