Multi-angle light scattering (MALS) is uniquely capable of measuring the absolute molar mass of transport proteins and their complexes in solution, providing direct assessment of their oligomeric state. Learn more by viewing our scientific poster.
Antibody-Drug Conjugates (ADCs) show much promise as effective therapeutics for cancer and other diseases, but they often exhibit an increased aggregation propensity compared to their unmodified counterparts due to non-specific interactions arising from attached drug and linker moieties. Light scattering offers multiple techniques for addressing the challenges of formulation screening, and characterizing both aggregates and propensity for aggregation. Learn more here.
Nanostructured Lipid Carriers (NLCs) increase skin hydration and re-enforce the natural lipid barrier of the skin. This study was conducted to prove the adsorption of silver ions onto the surface of NLCs using Asymmetric-Flow Field Flow Fractionation coupled with Multi Angle Light Scattering (AF4-MALS). Learn more by viewing our scientific poster.
In this study we used the Wyatt Eclipse Field Flow Fractionation System (aFFFF) to fractionate native alternan and multi-angle light scattering (MALS) coupled with a refractive index detector to measure its molar mass and size and compared these results to traditional SEC-MALS. The results show that native alternan was highly polydisperse, with a weight average molar mass of 50 million Da and an RMS radius of 45 nm. Learn more by viewing our scientific poster.
To understand the effects of release, exposure and transport of Engineered nanoparticles (NPs) and to evaluate possible risks for humans, selective and sensitive analytical methods are necessary in order to detect these materials in aerosols or liquid solutions. Read more about our NP analysis data obtained from AF4 combined with inductively coupled plasma mass spectrometry (ICPMS).
In this scientific paper, we studied the power relationship of radius of gyration and molecular weight, and viscometric radius and molecular weight of hyperbranched polyols. Learn more here.
Over-expression, or lack of clearance of amyloid β will result in the formation of insoluble protein fibers which bundle together to form amyloid plaques, a hallmark of the neurodegenerative disorder known as Alzheimer’s disease. Read about how we used analytical ultracentrifugation and size exclusion chromatography coupled with multi-angle laser light scattering to characterize various amyloid β preparations with the ultimate goal of being to correlate those results with the results of in-vitro toxicity assays and cell binding studies.
Size exclusion chromatography (SEC) coupled with MALS, UV, and refractive index (RI) detection of a transmembrane protein solubilized in detergent reveals the mass of the protein complex and can distinguish the protein mass from the associated detergent micelle.
An SEC assay has been developed to characterize three protein antigens of interest. Forced oligomerization experiments were performed to understand assay’s stability indications. Presented data revealed differences among protein antigens in their response to pH and ionic strength.
Development of a successful drug candidate requires extensive analysis of the product. Analytical tests fall into broad categories of characterization and comparability assays. This work describes the application of static and dynamic light scattering techniques for the characterization of a recombinant protein as a drug candidate.
Particle size analysis of environmental colloids with Flow-FFF has become a common practice. But the heterogeneous and non-spherical nature of environmental particles demands a characterization in terms of particle size and shape. Determining particle shape is possible with the combination of MALS and FFF.
This application note presents results obtained using an experimental set-up consisting of an HPLC pump, a sample injector, a multi-angle light scattering (MALS) photometer, a differential refractive index (DRI) detector, and an SEC guard column.
