March 29, 2023

AN6002: Automated Measurements of Electrophoretic Mobility

An HPLC system equipped with an autosampler enables unattended, automated measurements of electrophoretic mobility µE and hydrodynamic radius Rh with the Mobius™ instrument for dynamic and electrophoretic light scattering. A pre-programmed sequence controls the timing of injections, while the auto-inject signal triggers an Event Schedule in Wyatt’s DYNAMICS® software to collect data automatically.

October 10, 2022

AN2004: Why and how to quantify AAV aggregates by FFF-MALS

This application note compares analytical separations by size exclusion chromatography (SEC) and field-flow fractionation (FFF), along with detection by UV, fluorescence, and multi-angle light scattering (MALS). The results demonstrate that FFF-MALS is the most appropriate method for quantifying all aggregates of AAV-mediated products, from dimer, trimer, and small oligomers to large aggregates.

March 2, 2022

E-Book: Biopharmaceutical characterization: Improve predictive screening

Biopharmaceuticals, comprising monoclonal antibodies, antibody-drug conjugates, recombinant or fusion proteins, and bispecifics, among others, have recently gained commercial success and are rapidly changing the therapeutic landscape. Due to their large size and complex structures, characterizing these biotherapeutics requires robust and demanding analytical techniques. Often, traditional methods used to characterize proteins fail to deliver on either the sensitivity required or the speed desired to accelerate the drug development pipeline.

February 7, 2022

AN9010: Characterizing lentiviral and gammaretroviral vectors by batch DLS and FFF-MALS

This note demonstrates the use of batch dynamic light scattering (DLS) and online multi-angle light scattering and dynamic light scattering in combination with field-flow fractionation (FFF-MALS-DLS) for characterizing large viral vectors such as lentivirus and gammaretrovirus. DLS in batch mode rapidly screens particle size distribution, concentration/titer and stability.